Real Time PCR Detection System
Real-time polymerase chain reaction, also called quantitative real time polymerase chain reaction (Q-PCR/qPCR/qrt-PCR) or kinetic polymerase chain reaction (KPCR), is a laboratory technique based on the PCR, which is used to amplify and simultaneously quantify a targeted DNA molecule. Sample quantitation by Real-Time PCR increases reliability of gene expression analyses, improve viral load assessment, and accelerates throughput in screening applications. Bio-Rad's real-time PCR detection systems are optical modules that allow target sequence via fluorescent detection chemistry.

Real Time PCR Detection System Guide:

Real-Time PCR Detection Systems

My IQ™2 CFX-96™
Excitation range 70–500 nm 470–545 nm 450–684 nm 450–650 nm
Light source Light-emitting diode (LED) array (48 blue-green LEDs) Tungsten-halogen lamp 6 LEDs in optics shuttle 5 LEDs in optics shuttle
Optical detection 2 photodiodes 12-bit CCD camera 6-photodiodes 5 photodiodes
Detection range 523–700 nm 515–585 nm 515–730 nm 515–690 nm
Multiplex capability Up to 2 targets Up to 2 targets Up to 5 targets Up to 4 targets
Gradient range 35–99°C 40–100°C 30–100°C 30–100°C
Maximum gradient span 16°C 25°C 24°C 24°C
Maximum ramp rate 2.5°C/sec 3.3°C/sec 5°C/sec 2.5°C/sec
Dimensions (W x D x H) 18 x 32 x 33 cm 29 x 58 x 39 cm 33 x 46 x 36 cm 33 x 46 x 36 cm
Weight 6.8 kg 17.6 kg 21.4 kg 21.4 kg
Base thermal cycler MJ Mini iCycler C1000 C1000

For more detail:


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